Vol. 7, Issue 1, Part A (2025)

Aim: This scoping review investigates the effects of supplemental enzymes on biofilm formation related to orthopedic implant infections, focusing on Staphylococcus epidermidis and Staphylococcus aureus, to enhance post-implantation recovery and reduce infection rates.

Materials and Methods: A systematic search was conducted across Web of Science, EMBASE, and Cochrane Central for studies published between 2012 and 2022. Using keywords related to enzymes, biofilms, and medical devices, we filtered for randomized controlled trials in English. A total of 918 articles were screened, resulting in 6 eligible studies after applying inclusion and exclusion criteria.

Results: The review highlighted various enzymatic strategies, particularly endolysins and polysaccharide depolymerases, which effectively disrupt biofilm integrity. One study demonstrated that a combination of endolysins significantly improved the efficacy of traditional antibiotics against S. aureus and S. epidermidis biofilms. These enzymes facilitate the degradation of protective biofilm matrices, thus enhancing susceptibility to treatment.

Conclusions: The findings indicate a promising trend in the use of supplemental enzymes to mitigate biofilm formation on orthopedic implants, suggesting potential improvements in patient outcomes. This review underscores the necessity for further research to refine enzymatic therapies and establish standardized treatment protocols to combat biofilm-related infections effectively.